Spallanzani

Finally, to determine the question, Spallanzani began an assiduous course of observations and experiments.

Spallanzani placed fluids, which easily became putrid, in glass tubes, which he then hermetically sealed and boiled.

Spallanzani knew nothing of these organisms; they were not discovered until many years after his death.

From Spallanzani to Schultze, there were no further experiments to prove or disprove spontaneous generation.